Daptomycin inhibits cell envelope synthesis by interfering with fluid membrane microdomains.

ABSTRACT: Daptomycin is a highly efficient last-resort antibiotic that targets the bacterial cell membrane. Despite its clinical importance, the exact mechanism by which daptomycin kills bacteria is not fully understood. Different experiments have led to different models, including (i) blockage of cell wall synthesis, (ii) membrane pore formation, and (iii) the generation of altered membrane curvature leading to aberrant recruitment of proteins. To determine which model is correct, we carried out a comprehensive mode-of-action study using the model organism Bacillus subtilis and different assays, including proteomics, ionomics, and fluorescence light microscopy. We found that daptomycin causes a gradual decrease in membrane potential but does not form discrete membrane po

SUBMITTER: Muller A 

PROVIDER: S-EPMC5111643 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature