Molecular analysis of the factorless internal ribosome entry site in Cricket Paralysis virus infection.

ABSTRACT: The dicistrovirus Cricket Paralysis virus contains a unique dicistronic RNA genome arrangement, encoding two main open reading frames that are driven by distinct internal ribosome entry sites (IRES). The intergenic region (IGR) IRES adopts an unusual structure that directly recruits the ribosome and drives translation of viral structural proteins in a factor-independent manner. While structural, biochemical, and biophysical approaches have provided mechanistic details into IGR IRES translation, these studies have been limited to in vitro systems and little is known about the behavior of these IRESs during infection. Here, we examined the role of previously characterized IGR IRES mutations on viral yield and translation in CrPV-infected Drosophila S2 cells. Using a recently generated infect

SUBMITTER: Kerr CH 

PROVIDER: S-EPMC5112510 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature