A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum.

ABSTRACT:

Background

Pyruvate kinase (Pyk) catalyzes the generation of pyruvate and ATP in glycolysis and functions as a key switch in the regulation of carbon flux distribution. Both the substrates and products of Pyk are involved in the tricarboxylic acid cycle, anaplerosis and energy anabolism, which places Pyk at a primary metabolic intersection. Pyks are highly conserved in most bacteria and lower eukaryotes. Corynebacterium glutamicum is an industrial workhorse for the production of various amino acids and organic acids. Although C. glutamicum was assumed to possess only one Pyk (pyk1, NCgl2008), NCgl2809 was annotated as a pyruvate kinase with an unknown role.

Results

Here, we identified that NCgl2809 was a novel pyruvate kinase (pyk2) in C. glutamicum. Complementation of the W

SUBMITTER: Chai X 

PROVIDER: S-EPMC5112673 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature