Modeling of Transmembrane Potential in Realistic Multicellular Structures before Electroporation.

ABSTRACT: Many approaches for studying the transmembrane potential (TMP) induced during the treatment of biological cells with pulsed electric fields have been reported. From the simple analytical models to more complex numerical models requiring significant computational resources, a gamut of methods have been used to recapitulate multicellular environments in silico. Cells have been modeled as simple shapes in two dimensions as well as more complex geometries attempting to replicate realistic cell shapes. In this study, we describe a method for extracting realistic cell morphologies from fluorescence microscopy images to generate the piecewise continuous mesh used to develop a finite element model in two dimensions. The preelectroporation TMP induced in tightly packed cells is analyzed for two set

SUBMITTER: Murovec T 

PROVIDER: S-EPMC5113126 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature