The impact of mechanical compression on cortical microtubules in Arabidopsis: a quantitative pipeline.

ABSTRACT: Exogenous mechanical perturbations on living tissues are commonly used to investigate whether cell effectors can respond to mechanical cues. However, in most of these experiments, the applied mechanical stress and/or the biological response are described only qualitatively. We developed a quantitative pipeline based on microindentation and image analysis to investigate the impact of a controlled and prolonged compression on microtubule behaviour in the Arabidopsis shoot apical meristem, using microtubule fluorescent marker lines. We found that a compressive stress, in the order of magnitude of turgor pressure, induced apparent microtubule bundling. Importantly, that response could be reversed several hours after the release of compression. Next, we tested the contribution of microtubule se

SUBMITTER: Louveaux M 

PROVIDER: S-EPMC5113706 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature