Automatic Dendritic Spine Quantification from Confocal Data with Neurolucida 360.

ABSTRACT: Determining the density and morphology of dendritic spines is of high biological significance given the role of spines in synaptic plasticity and in neurodegenerative and neuropsychiatric disorders. Precise quantification of spines in three dimensions (3D) is essential for understanding the structural determinants of normal and pathological neuronal function. However, this quantification has been restricted to time- and labor-intensive methods such as electron microscopy and manual counting, which have limited throughput and are impractical for studies of large samples. While there have been some automated software packages that quantify spine number, they are limited in terms of their characterization of spine structure. This unit presents methods for objective dendritic spine morphometri

SUBMITTER: Dickstein DL 

PROVIDER: S-EPMC5113738 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature