Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion.

ABSTRACT: Insulin-secreting beta cells play an essential role in maintaining physiological blood glucose levels, and their dysfunction leads to the development of diabetes. To elucidate the signalling events regulating insulin secretion, we applied a recently developed phosphoproteomics workflow. We quantified the time-resolved phosphoproteome of murine pancreatic cells following their exposure to glucose and in combination with small molecule compounds that promote insulin secretion. The quantitative phosphoproteome of 30,000 sites clustered into three main groups in concordance with the modulation of the three key kinases: PKA, PKC and CK2A. A high-resolution time course revealed key novel regulatory sites, revealing the importance of methyltransferase DNMT3A phosphorylation in the glucose respons

SUBMITTER: Sacco F 

PROVIDER: S-EPMC5114537 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature