TRNA-mediated codon-biased translation in mycobacterial hypoxic persistence.

ABSTRACT: Microbial pathogens adapt to the stress of infection by regulating transcription, translation and protein modification. We report that changes in gene expression in hypoxia-induced non-replicating persistence in mycobacteria-which models tuberculous granulomas-are partly determined by a mechanism of tRNA reprogramming and codon-biased translation. Mycobacterium bovis BCG responded to each stage of hypoxia and aerobic resuscitation by uniquely reprogramming 40 modified ribonucleosides in tRNA, which correlate with selective translation of mRNAs from families of codon-biased persistence genes. For example, early hypoxia increases wobble cmo⁵U in tRNA^Thr(UGU), which parallels translation of transcripts enriched in its cognate codon, ACG, including the DosR master regulat

SUBMITTER: Chionh YH 

PROVIDER: S-EPMC5114619 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature