Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis.

ABSTRACT: Peripheral CD27⁺ memory B-cells become quantitatively reduced and dysfunctional in patients with cirrhosis through poorly characterized mechanisms. We hypothesized that the disappearance of CD27⁺ memory B-cells results from enhanced sensitivity to apoptosis caused by exposure to gut microbial translocation products. Using isolated naïve and memory B-cells from patients with cirrhosis and age-matched controls, ex vivo and activation-induced sensitivity to Fas-mediated apoptosis was assessed under relevant experimental conditions. We observed differential expression of CD95(Fas) in CD27⁺ B-cells from cirrhotic patients that was inversely correlated with peripheral CD27⁺ B-cell frequency. While memory B-cells from cirrhotic patients were resistant t

SUBMITTER: Chang LY 

PROVIDER: S-EPMC5114671 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature