MicroRNA let-7, T Cells, and Patient Survival in Colorectal Cancer.

ABSTRACT: Experimental evidence suggests that the let-7 family of noncoding RNAs suppresses adaptive immune responses, contributing to immune evasion by the tumor. We hypothesized that the amount of let-7a and let-7b expression in colorectal carcinoma might be associated with limited T-lymphocyte infiltrates in the tumor microenvironment and worse clinical outcome. Utilizing the molecular pathological epidemiology resources of 795 rectal and colon cancers in two U.S.-nationwide prospective cohort studies, we measured tumor-associated let-7a and let-7b expression levels by quantitative reverse-transcription PCR, and CD3⁺, CD8⁺, CD45RO (PTPRC)⁺, and FOXP3⁺ cell densities by tumor tissue microarray immunohistochemistry and computer-assisted image analysis. Lo

SUBMITTER: Dou R 

PROVIDER: S-EPMC5115629 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature