Role of the σ⁵⁴ Activator Interacting Domain in Bacterial Transcription Initiation.

ABSTRACT: Bacterial sigma factors are subunits of RNA polymerase that direct the holoenzyme to specific sets of promoters in the genome and are a central element of regulating transcription. Most polymerase holoenzymes open the promoter and initiate transcription rapidly after binding. However, polymerase containing the members of the σ⁵⁴ family must be acted on by a transcriptional activator before DNA opening and initiation occur. A key domain in these transcriptional activators forms a hexameric AAA+ ATPase that acts through conformational changes brought on by ATP hydrolysis. Contacts between the transcriptional activator and σ⁵⁴ are primarily made through an N-terminal σ⁵⁴ activator interacting domain (AID). To better understand this mechanism of bacterial trans

SUBMITTER: Siegel AR 

PROVIDER: S-EPMC5116005 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature