Carbene footprinting accurately maps binding sites in protein-ligand and protein-protein interactions.

ABSTRACT: Specific interactions between proteins and their binding partners are fundamental to life processes. The ability to detect protein complexes, and map their sites of binding, is crucial to understanding basic biology at the molecular level. Methods that employ sensitive analytical techniques such as mass spectrometry have the potential to provide valuable insights with very little material and on short time scales. Here we present a differential protein footprinting technique employing an efficient photo-activated probe for use with mass spectrometry. Using this methodology the location of a carbohydrate substrate was accurately mapped to the binding cleft of lysozyme, and in a more complex example, the interactions between a 100 kDa, multi-domain deubiquitinating enzyme, USP5 and a diubiqu

SUBMITTER: Manzi L 

PROVIDER: S-EPMC5116083 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature