Characterization of GABA_A receptor ligands with automated patch-clamp using human neurons derived from pluripotent stem cells.

ABSTRACT:

Introduction

Automated patch clamp is a recent but widely used technology to assess pre-clinical drug safety. With the availability of human neurons derived from pluripotent stem cells, this technology can be extended to determine CNS effects of drug candidates, especially those acting on the GABA_A receptor.

Methods

iCell Neurons (Cellular Dynamics International, A Fujifilm Company) were cultured for ten days and analyzed by patch clamp in the presence of agonist GABA or in combination with positive allosteric GABA_A receptor modulators. Both efficacy and affinity were determined. In addition, mRNA of GABA_A receptor subunits were quantified by qRT-PCR.

Results

We have shown that iCell Neurons are compatible with the IonFlux microfluidic

SUBMITTER: Yuan NY 

PROVIDER: S-EPMC5116268 | biostudies-literature | 2016 Nov - Dec

REPOSITORIES: biostudies-literature