Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.

ABSTRACT: With readily available transcriptome-wide data, understanding the role of each expressed gene is an essential next step. Although RNAi technologies allow for genome-wide screens in cell culture, these approaches cannot replace strategies for discovery in the embryo. Here we present, for the first time, a knockdown screen in mouse preimplantation embryos. Early mammalian development encompasses dynamic cellular, molecular and epigenetic events that are largely conserved from mouse to man. We assayed 712 genes for requirements during preimplantation. We identified 59 genes required for successful development or outgrowth and implantation. We have characterized each phenotype and revealed cellular, molecular, and lineage specific defects following knockdown of transcript. Induced network anal

SUBMITTER: Cui W 

PROVIDER: S-EPMC5116644 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature