Establishing Proximal and Distal Regional Identities in Murine and Human Tissue-Engineered Lung and Trachea.

ABSTRACT: The cellular and molecular mechanisms that underpin regeneration of the human lung are unknown, and the study of lung repair has been impeded by the necessity for reductionist models that may exclude key components. We hypothesized that multicellular epithelial and mesenchymal cell clusters or lung organoid units (LuOU) could be transplanted to recapitulate proximal and distal cellular structures of the native lung and airways. Transplantation of LuOU resulted in the growth of tissue-engineered lung (TELu) that contained the necessary cell types consistent with native adult lung tissue and demonstrated proliferative cells at 2 and 4 weeks. This technique recapitulated important elements of both mouse and human lungs featuring key components of both the proximal and distal lung regions. Whe

SUBMITTER: Trecartin A 

PROVIDER: S-EPMC5116661 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature