Classification of Tandem Mass Spectra for Identification of N- and O-linked Glycopeptides.

ABSTRACT: Analysis of intact glycopeptides by mass spectrometry is essential to determining the microheterogeneity of protein glycosylation. Higher-energy collisional dissociation (HCD) fragmentation of glycopeptides generates mono- or disaccharide ions called oxonium ions that carry information about the structure of the fragmented glycans. Here, we investigated the link between glycan structures and the intensity of oxonium ions in the spectra of glycopeptides and utilized this information to improve the identification of glycopeptides in biological samples. Tandem spectra of glycopeptides from fetuin, glycophorin A, ovalbumin and gp120 tryptic digests were used to build a spectral database of N- and O-linked glycopeptides. Logistic regression was applied to this database to develop model to disti

SUBMITTER: Toghi Eshghi S 

PROVIDER: S-EPMC5116676 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature