EGF-coated gold nanoparticles provide an efficient nano-scale delivery system for the molecular radiotherapy of EGFR-positive cancer.

ABSTRACT: Purpose Radiolabeled antibodies and peptides hold promise for molecular radiotherapy but are often limited by a low payload resulting in inadequate delivery of radioactivity to tumour tissue and, therefore, modest therapeutic effect. We developed a facile synthetic method of radiolabeling indium-111 (¹¹¹In) to epidermal growth factor (EGF)-gold nanoparticles (¹¹¹In-EGF-Au NP) with a high payload. Materials and methods EGF-Au NP were prepared via an interaction between gold and the disulphide bonds of EGF and radiolabeled using ¹¹¹InCl₃. Targeting efficiency was investigated by quantitating internalized radioactivity and by confocal imaging following exposure of MDA-MB-468 (1.3 × 10⁶ EGFR/cell) and MCF-7 (10⁴ EGFR/cell) cells

SUBMITTER: Song L 

PROVIDER: S-EPMC5116916 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature