Up-regulation of miR-203 expression induces endothelial inflammatory response: Potential role in preeclampsia.

ABSTRACT:

Problem

To determine whether miR-203 mediates endothelial inflammatory response in preeclampsia.

Method of study

Maternal vessel miR-203 expression was assessed by in situ hybridization. Suppressor of cytokine signaling-3 (SOCS-3) and ICAM expression was determined by immunostaining. Subcutaneous fat tissue sections from normal and preeclamptic pregnant women were used. miR-203-induced inflammatory response was evaluated by the measurements of IL-6, IL-8, ICAM, and VCAM expression and production and neutrophil adhesion in the endothelial cells (EC) transfected with miR-203 precursor, pre-miR-203. SOCS3 expression was also determined.

Results

Up-regulation of miR-203 and ICAM expression and down-regulation of SOCS-3 expression were demonstrated in maternal vessel endot

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5118090 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature