Enamel ribbons, surface nodules, and octacalcium phosphate in C57BL/6 Amelx^-/- mice and Amelx^+/- lyonization.

ABSTRACT:

Background

Amelogenin is required for normal enamel formation and is the most abundant protein in developing enamel.

Methods

Amelx^+/+, Amelx^+/- , and Amelx^-/- molars and incisors from C57BL/6 mice were characterized using RT-PCR, Western blotting, dissecting and light microscopy, immunohistochemistry (IHC), transmission electron microscopy (TEM), scanning electron microscopy (SEM), backscattered SEM (bSEM), nanohardness testing, and X-ray diffraction.

Results

No amelogenin protein was detected by Western blot analyses of enamel extracts from Amelx^-/- mice. Amelx^-/- incisor enamel averaged 20.3 ± 3.3 μm in thickness, or only

SUBMITTER: Hu Y 

PROVIDER: S-EPMC5118209 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature