Extending a Single Residue Switch for Abbreviating Catalysis in Plant ent-Kaurene Synthases.

ABSTRACT: Production of ent-kaurene as a precursor for important signaling molecules such as the gibberellins seems to have arisen early in plant evolution, with corresponding cyclase(s) present in all land plants (i.e., embryophyta). The relevant enzymes seem to represent fusion of the class II diterpene cyclase that produces the intermediate ent-copalyl diphosphate (ent-CPP) and the subsequently acting class I diterpene synthase that produces ent-kaurene, although the bifunctionality of the ancestral gene is only retained in certain early diverging plants, with gene duplication and sub-functionalization leading to distinct ent-CPP synthases and ent-kaurene synthases (KSs) generally observed. This evolutionary scenario implies that plant KSs should have con

SUBMITTER: Jia M 

PROVIDER: S-EPMC5118566 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature