Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi.

ABSTRACT: Biofilms enhance rates of gene exchange, access to specific nutrients, and cell survivability. Haloarchaea in Deep Lake, Antarctica, are characterized by high rates of intergenera gene exchange, metabolic specialization that promotes niche adaptation, and are exposed to high levels of UV-irradiation in summer. Halorubrum lacusprofundi from Deep Lake has previously been reported to form biofilms. Here we defined growth conditions that promoted the formation of biofilms and used microscopy and enzymatic digestion of extracellular material to characterize biofilm structures. Extracellular DNA was found to be critical to biofilms, with cell surface proteins and quorum sensing also implicated in biofilm formation. Quantitative proteomics was used to define pathways and cellular processes involv

SUBMITTER: Liao Y 

PROVIDER: S-EPMC5118699 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature