Development of a rapid screen for the endodermal differentiation potential of human pluripotent stem cell lines.

ABSTRACT: A challenge facing the human pluripotent stem cell (hPSC) field is the variability observed in differentiation potential of hPSCs. Variability can lead to time consuming and costly optimisation to yield the cell type of interest. This is especially relevant for the differentiation of hPSCs towards the endodermal lineages. Endodermal cells have the potential to yield promising new knowledge and therapies for diseases affecting multiple organ systems, including lung, thymus, intestine, pancreas and liver, as well as applications in regenerative medicine and toxicology. Providing a means to rapidly, cheaply and efficiently assess the differentiation potential of multiple hPSCs is of great interest. To this end, we have developed a rapid small molecule based screen to assess the endodermal pot

SUBMITTER: Siller R 

PROVIDER: S-EPMC5118706 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature