Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley.

ABSTRACT: Different plant cultivars of the same genus and species can exhibit vastly different genetic transformation efficiencies. However, the genetic factors underlying these differences in transformation rate remain largely unknown. In barley, 'Golden Promise' is one of a few cultivars reliable for Agrobacterium-mediated transformation. By contrast, cultivar 'Haruna Nijo' is recalcitrant to genetic transformation. We identified genomic regions of barley important for successful transformation with Agrobacterium, utilizing the 'Haruna Nijo' × 'Golden Promise' F₂ generation and genotyping by 124 genome-wide SNP markers. We observed significant segregation distortions of these markers from the expected 1:2:1 ratio toward the 'Golden Promise'-type in regions of chromosomes 2H and 3H, indi

SUBMITTER: Hisano H 

PROVIDER: S-EPMC5118740 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature