Heterogeneous antigenic properties of the porcine reproductive and respiratory syndrome virus nucleocapsid.

ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterivirus responsible for a widespread contagious disease of domestic pigs with high economic impact. Switzerland is one of the rare PRRSV-free countries in Europe, although sporadic outbreaks have occurred in the past. The PRRSV isolate IVI-1173 from the short outbreak in Switzerland in 2012 was entirely sequenced, and a functional full-length cDNA clone was constructed. Genetic and antigenic characterization of IVI-1173 revealed the importance of amino acid 90 of the nucleocapsid protein N as part of a conformational epitope. IVI-1173 was not detected by SDOW17, a monoclonal antibody against N widely used to detect PRRSV-infected cells. Substitution of alanine at position 90 of N [N(A₉₀)] with a threonine [N(T

SUBMITTER: Rappe JC 

PROVIDER: S-EPMC5118883 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature