Simulation study of the ability of a computationally-designed peptide to recognize target tRNA^Lys3 and other decoy tRNAs.

ABSTRACT: In this paper, we investigate the ability of our computationally-designed peptide, Pept10 (PNWNGNRWLNNCLRG), to recognize the anticodon stem and loop (ASL) domain of the hypermodified tRNA^Lys3 (mcm⁵ s² U₃₄ ,ms² t⁶ A₃₇ ), a reverse transcription primer of HIV replication. Five other ASLs, the singly modified ASL^Lys3 (ms² t⁶ A₃₇ ), ASL^Lys3 (s² U₃₄ ), ASL^Lys3 (Ψ₃₉ ), ASL^Lys1,2 (t⁶ A₃₇ ), and ASL^Glu (s² U₃₄ ), were used as decoys. Explicit-solvent atomistic molecular dynamics simulations were performed to examine the process of binding of Pept10 with the t

SUBMITTER: Xiao X 

PROVIDER: S-EPMC5119567 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature