Levels of DNA methylation and transcript accumulation in leaves of transgenic maize varieties.

ABSTRACT:

Background

Prior to their release in the environment, transgenic crops are examined for their health and environmental safety. In addition, transgene expression needs to be consistent in order to express the introduced trait (e.g. insecticidal and/or herbicide tolerance). Moreover, data on expression levels for GM events are usually required for approval, but these are rarely disclosed or they are considered insufficient. On the other hand, biosafety regulators do not consider epigenetic regulation (e.g. DNA methylation, ncRNAs and histone modifications), which are broadly known to affect gene expression, within their risk assessment analyses. Here we report the results of a DNA methylation (bisulfite sequencing) and transgene transcript accumulation (RT-qPCR) analysis of four Bt-e

SUBMITTER: Vilperte V 

PROVIDER: S-EPMC5120055 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature