NCR1 Expression Identifies Canine Natural Killer Cell Subsets with Phenotypic Similarity to Human Natural Killer Cells.

ABSTRACT: Canines spontaneously develop many cancers similar to humans - including osteosarcoma, leukemia, and lymphoma - offering the opportunity to study immune therapies in a genetically heterogeneous and immunocompetent environment. However, a lack of antibodies recognizing canine NK cell markers has resulted in suboptimal characterization and unknown purity of NK cell products, hindering the development of canine models of NK cell adoptive immunotherapy. To this end, we generated a novel antibody to canine NCR1 (NKp46), the putative species-wide marker of NK cells, enabling purification of NK cells for further characterization. We demonstrate that CD3^-/NKp46⁺ cells in healthy and osteosarcoma-bearing canines have phenotypic similarity to human CD3^-/NKp46^+</

SUBMITTER: Foltz JA 

PROVIDER: S-EPMC5120128 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature