Toward a new paradigm of DNA writing using a massively parallel sequencing platform and degenerate oligonucleotide.

ABSTRACT: All synthetic DNA materials require prior programming of the building blocks of the oligonucleotide sequences. The development of a programmable microarray platform provides cost-effective and time-efficient solutions in the field of data storage using DNA. However, the scalability of the synthesis is not on par with the accelerating sequencing capacity. Here, we report on a new paradigm of generating genetic material (writing) using a degenerate oligonucleotide and optomechanical retrieval method that leverages sequencing (reading) throughput to generate the desired number of oligonucleotides. As a proof of concept, we demonstrate the feasibility of our concept in digital information storage in DNA. In simulation, the ability to store data is expected to exponentially increase with increa

SUBMITTER: Hwang B 

PROVIDER: S-EPMC5120280 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature