Vertical flow array chips reliably identify cell types from single-cell mRNA sequencing experiments.

ABSTRACT: Single-cell mRNA sequencing offers an unbiased approach to dissecting cell types as functional units in multicellular tissues. However, highly reliable cell typing based on single-cell gene expression analysis remains challenging because of the lack of methods for efficient sample preparation for high-throughput sequencing and evaluating the statistical reliability of the acquired cell types. Here, we present a highly efficient nucleic reaction chip (a vertical flow array chip (VFAC)) that uses porous materials to reduce measurement noise and improve throughput without a substantial increase in reagent. We also present a probabilistic evaluation method for cell typing depending on the amount of measurement noise. Applying the VFACs to 2580 monocytes provides 1967 single-cell expressions fo

SUBMITTER: Shirai M 

PROVIDER: S-EPMC5120284 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature