Time-course microarray analysis for identifying candidate genes involved in obesity-associated pathological changes in the mouse colon.

ABSTRACT:

Background

Obesity is known to increase the risk of colorectal cancer. However, mechanisms underlying the pathogenesis of obesity-induced colorectal cancer are not completely understood. The purposes of this study were to identify differentially expressed genes in the colon of mice with diet-induced obesity and to select candidate genes as early markers of obesity-associated abnormal cell growth in the colon.

Methods

C57BL/6N mice were fed normal diet (11% fat energy) or high-fat diet (40% fat energy) and were euthanized at different time points. Genome-wide expression profiles of the colon were determined at 2, 4, 8, and 12 weeks. Cluster analysis was performed using expression data of genes showing log₂ fold change of ≥1 or ≤-1 (twofold change), based on time-de

SUBMITTER: Bae YJ 

PROVIDER: S-EPMC5120484 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature