Alteration in cellular turnover and progenitor cell population in lacrimal glands from thrombospondin 1^-/- mice, a model of dry eye.

ABSTRACT: The purpose of this study was to investigate the changes that occur in the lacrimal glands (LGs) in female thrombospondin 1 knockout (TSP1^-/-) mice, a mouse model of the autoimmune disease Sjogren's syndrome. The LGs of 4, 12, and 24 week-old female TSP1^-/- and C57BL/6J (wild type, WT) mice were used. qPCR was performed to measure cytokine expression. To study the architecture, LG sections were stained with hematoxylin and eosin. Cell proliferation was measured using bromo-deoxyuridine and immunohistochemistry. Amount of CD47 and stem cell markers was analyzed by western blot analysis and location by immunofluorescence microscopy. Expression of stem cell transcription factors was performed using Mouse Stem Cell Transcription Factors RT² Profiler PCR Array.

SUBMITTER: Shatos MA 

PROVIDER: S-EPMC5121046 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature