Human airway organoid engineering as a step toward lung regeneration and disease modeling.

ABSTRACT: Organoids represent both a potentially powerful tool for the study cell-cell interactions within tissue-like environments, and a platform for tissue regenerative approaches. The development of lung tissue-like organoids from human adult-derived cells has not previously been reported. Here we combined human adult primary bronchial epithelial cells, lung fibroblasts, and lung microvascular endothelial cells in supportive 3D culture conditions to generate airway organoids. We demonstrate that randomly-seeded mixed cell populations undergo rapid condensation and self-organization into discrete epithelial and endothelial structures that are mechanically robust and stable during long term culture. After condensation airway organoids generate invasive multicellular tubular structures that recapit

SUBMITTER: Tan Q 

PROVIDER: S-EPMC5121055 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature