Comprehensive characterization of genomic instability in pluripotent stem cells and their derived neuroprogenitor cell lines.

ABSTRACT: The genomic integrity of two human pluripotent stem cells and their derived neuroprogenitor cell lines was studied, applying a combination of high-resolution genetic methodologies. The usefulness of combining array-comparative genomic hybridization (aCGH) and multiplex fluorescence in situ hybridization (M-FISH) techniques should be delineated to exclude/detect a maximum of possible genomic structural aberrations. Interestingly, in parts different genomic imbalances at chromosomal and subchromosomal levels were detected in pluripotent stem cells and their derivatives. Some of the copy number variations were inherited from the original cell line, whereas other modifications were presumably acquired during the differentiation and manipulation procedures. These results underline the necessity

SUBMITTER: Lopez Corrales NL 

PROVIDER: S-EPMC5121198 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature