A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.

ABSTRACT: Molecular diagnostic measurements are currently underpinned by the polymerase chain reaction (PCR). There are also a number of alternative nucleic acid amplification technologies, which unlike PCR, work at a single temperature. These 'isothermal' methods, reportedly offer potential advantages over PCR such as simplicity, speed and resistance to inhibitors and could also be used for quantitative molecular analysis. However there are currently limited mechanisms to evaluate their quantitative performance, which would assist assay development and study comparisons. This study uses a sexually transmitted infection diagnostic model in combination with an adapted metric termed isothermal doubling time (IDT), akin to PCR efficiency, to compare quantitative PCR and quantitative loop-mediated isoth

SUBMITTER: Nixon GJ 

PROVIDER: S-EPMC5121211 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature