Gene regulatory networks in neural cell fate acquisition from genome-wide chromatin association of Geminin and Zic1.

ABSTRACT: Neural cell fate acquisition is mediated by transcription factors expressed in nascent neuroectoderm, including Geminin and members of the Zic transcription factor family. However, regulatory networks through which this occurs are not well defined. Here, we identified Geminin-associated chromatin locations in embryonic stem cells and Geminin- and Zic1-associated locations during neural fate acquisition at a genome-wide level. We determined how Geminin deficiency affected histone acetylation at gene promoters during this process. We integrated these data to demonstrate that Geminin associates with and promotes histone acetylation at neurodevelopmental genes, while Geminin and Zic1 bind a shared gene subset. Geminin- and Zic1-associated genes exhibit embryonic nervous system-enriched express

SUBMITTER: Sankar S 

PROVIDER: S-EPMC5121602 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature