V(D)J recombination process and the Pre-B to immature B-cells transition are altered in Fanca^-/- mice.

ABSTRACT: B-lymphocytes in the bone marrow (BM) must generate a functional B-cell receptor and overcome the negative selection induced by reactivity with autoantigens. Two rounds of DNA recombination are required for the production of functional immunoglobulin heavy (Ig-HCs) and light (LCs) chains necessary for the continuation of B-lymphocyte development in the BM. Both rounds depend on the joint action of recombination activating gene-1 (RAG-1) and RAG-2 endonucleases with the DNA non-homologous end-joining pathway. Loss of the FANC gene leads to the chromosome breakage and cancer predisposition syndrome Fanconi anemia. Because the FANC proteins are involved in certain aspects of the recombination process, we sought to determine the impact of the FANC pathway on the Ig diversification process usin

SUBMITTER: Nguyen TV 

PROVIDER: S-EPMC5121645 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature