MQC: A Heuristic Quality-Control Metric for High-Throughput Drug Combination Screening.

ABSTRACT: Quality control (QC) metrics are critical in high throughput screening (HTS) platforms to ensure reliability and confidence in assay data and downstream analyses. Most reported HTS QC metrics are designed for plate level or single well level analysis. With the advent of high throughput combination screening there is a need for QC metrics that quantify the quality of combination response matrices. We introduce a predictive, interpretable, matrix-level QC metric, mQC, based on a mix of data-derived and heuristic features. mQC accurately reproduces the expert assessment of combination response quality and correctly identifies unreliable response matrices that can lead to erroneous or misleading characterization of synergy. When combined with the plate-level QC metric, Z', mQC provides a more

SUBMITTER: Chen L 

PROVIDER: S-EPMC5121902 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature