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Functional Analysis of the Nitrogen Metabolite Repression Regulator Gene nmrA in Aspergillus flavus.


ABSTRACT: In Aspergillus nidulans, the nitrogen metabolite repression (NMR) regulator NmrA plays a major role in regulating the activity of the GATA transcription factor AreA during nitrogen metabolism. However, the function of nmrA in A. flavus has not been previously studied. Here, we report the identification and functional analysis of nmrA in A. flavus. Our work showed that the amino acid sequences of NmrA are highly conserved among Aspergillus species and that A. flavus NmrA protein contains a canonical Rossmann fold motif. Deletion of nmrA slowed the growth of A. flavus but significantly increased conidiation and sclerotia production. Moreover, seed infection experiments indicated that nmrA is required for the invasive virulence of A. flavus. In addition, the ?nmrA mutant showed increased sensitivity to rapamycin and methyl methanesulfonate, suggesting that nmrA could be responsive to target of rapamycin signaling and DNA damage. Furthermore, quantitative real-time reverse transcription polymerase chain reaction analysis suggested that nmrA might interact with other nitrogen regulatory and catabolic genes. Our study provides a better understanding of NMR and the nitrogen metabolism network in fungi.

SUBMITTER: Han X 

PROVIDER: S-EPMC5122588 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Functional Analysis of the Nitrogen Metabolite Repression Regulator Gene <i>nmrA</i> in <i>Aspergillus flavus</i>.

Han Xiaoyun X   Qiu Mengguang M   Wang Bin B   Yin Wen-Bing WB   Nie Xinyi X   Qin Qiuping Q   Ren Silin S   Yang Kunlong K   Zhang Feng F   Zhuang Zhenhong Z   Wang Shihua S  

Frontiers in microbiology 20161125


In <i>Aspergillus nidulans</i>, the nitrogen metabolite repression (NMR) regulator NmrA plays a major role in regulating the activity of the GATA transcription factor AreA during nitrogen metabolism. However, the function of <i>nmrA</i> in <i>A. flavus</i> has not been previously studied. Here, we report the identification and functional analysis of <i>nmrA</i> in <i>A. flavus</i>. Our work showed that the amino acid sequences of NmrA are highly conserved among <i>Aspergillus</i> species and tha  ...[more]

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