Engineering a wild-type diploid Saccharomyces cerevisiae strain for second-generation bioethanol production.

ABSTRACT:

Background

The cost-effective production of second-generation bioethanol, which is made from lignocellulosic materials, has to face the following two problems: co-fermenting xylose with glucose and enhancing the strain's tolerance to lignocellulosic inhibitors. Based on our previous study, the wild-type diploid Saccharomyces cerevisiae strain BSIF with robustness and good xylose metabolism genetic background was used as a chassis for constructing efficient xylose-fermenting industrial strains. The performance of the resulting strains in the fermentation of media with sugars and hydrolysates was investigated.

Results

The following two novel heterologous genes were integrated into the genome of the chassis cell: the mutant MGT05196^N360F, which encodes

SUBMITTER: Li H 

PROVIDER: S-EPMC5122614 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature