The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization.

ABSTRACT: Vascular permeability and neovascularization are implicated in many diseases including retinopathies and diabetic wound healing. Robo4 is an endothelial-specific transmembrane receptor that stabilizes the vasculature, as shown in Robo4^-/- mice that develop hyperpermeability, but how Robo4 signals remained unclear. Here we show that Robo4 deletion enhances permeability and revascularization in oxygen-induced retinopathy (OIR) and accelerates cutaneous wound healing. To determine Robo4 signalling pathways, we generated transgenic mice expressing a truncated Robo4 lacking the cytoplasmic domain (Robo4ΔCD). Robo4ΔCD expression is sufficient to prevent permeability, and inhibits OIR revascularization and wound healing in Robo4^-/- mice. Mechanistically, Robo4 does not affec

SUBMITTER: Zhang F 

PROVIDER: S-EPMC5123080 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature