Adipocyte-specific Hypoxia-inducible gene 2 promotes fat deposition and diet-induced insulin resistance.

ABSTRACT:

Objective

Adipose tissue relies on lipid droplet (LD) proteins in its role as a lipid-storing endocrine organ that controls whole body metabolism. Hypoxia-inducible Gene 2 (Hig2) is a recently identified LD-associated protein in hepatocytes that promotes hepatic lipid storage, but its role in the adipocyte had not been investigated. Here we tested the hypothesis that Hig2 localization to LDs in adipocytes promotes adipose tissue lipid deposition and systemic glucose homeostasis.

Method

White and brown adipocyte-deficient (Hig2^fl/fl × Adiponection cre+) and selective brown/beige adipocyte-deficient (Hig2^fl/fl × Ucp1 cre+) mice were generated to investigate the role of Hig2 in adipose depots. Additionally, we used multiple housing temperatures to investig

SUBMITTER: DiStefano MT 

PROVIDER: S-EPMC5123203 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature