RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk.

ABSTRACT:

Background

In this work we aimed at sequencing and assembling the goat milk transcriptome corresponding at colostrum and 120 days of lactation. To reconstruct transcripts we used both the genome as reference, and a de novo assembly approach. Additionally, we aimed at identifying the differentially expressed genes (DEGs) between the two lactation stages and at analyzing the expression of genes involved in oligosaccharides metabolism.

Results

A total of 44,635 different transcripts, organized in 33,757 tentative genes, were obtained using the goat genome as reference. A significant sequence similarity match was found for 40,353 transcripts (90%) against the NCBI NT and for 35,701 (80%) against the NR databases. 68% and 69% of the de novo assembled transcripts, in colostrum and

SUBMITTER: Crisa A 

PROVIDER: S-EPMC5123407 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature