Valproic acid protects against haemorrhagic shock-induced signalling changes via PPARγ activation in an in vitro model.

ABSTRACT:

Background and purpose

Valproic acid (VPA), a widely used epilepsy and bipolar disorder treatment, provides acute protection against haemorrhagic shock-induced mortality in a range of in vivo models through an unknown mechanism. In the liver, this effect occurs with a concomitant protection against a decrease in GSK3β-Ser(9) phosphorylation. Here, we developed an in vitro model to investigate this protective effect of VPA and define a molecular mechanism.

Experimental approach

The human hepatocarcinoma cell line (Huh7) was exposed to conditions occurring during haemorrhagic shock (hypoxia, hypercapnia and hypothermia) to investigate the changes in GSK3β-Ser(9) phosphorylation for a 4 h period following treatment with VPA, related congeners, PPAR agonists, antagonists and siR

SUBMITTER: Zuckermann AM 

PROVIDER: S-EPMC5123713 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature