Isolation of human trophoblastic extracellular vesicles and characterization of their cargo and antiviral activity.

ABSTRACT:

Introduction

Primary human trophoblasts release a repertoire of extracellular vesicles (EVs). Among them are nano-sized exosomes, which we found to suppress the replication of a wide range of diverse viruses. These exosomes contain trophoblastic microRNAs (miRNAs) that are expressed from the chromosome 19 miRNA cluster and exhibit antiviral properties. Here, we report our investigation of the cargo of placental EVs, focusing on the composition and the antiviral properties of exosomes, microvesicles, and apoptotic blebs.

Methods

We isolated EVs using ultracentrifugation and defined their purity using immunoblotting, electron microscopy, and nanoparticle tracking. We used liquid chromatography-electrospray ionization-mass spectrometry, protein mass spectrometry, and miRNA TaqM

SUBMITTER: Ouyang Y 

PROVIDER: S-EPMC5123854 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature