Newly synthesized and recycling pools of the apical protein gp135 do not occupy the same compartments.

ABSTRACT: Polarized epithelial cells sort newly synthesized and recycling plasma membrane proteins into distinct trafficking pathways directed to either the apical or basolateral membrane domains. While the trans-Golgi network is a well-established site of protein sorting, increasing evidence indicates a key role for endosomes in the initial trafficking of newly synthesized proteins. Both basolateral and apical proteins have been shown to traverse endosomes en route to the plasma membrane. In particular, apical proteins traffic through either subapical early or recycling endosomes. Here we use the SNAP tag system to analyze the trafficking of the apical protein gp135, also known as podocalyxin. We show that newly synthesized gp135 traverses the apical recycling endosome, but not the apical early end

SUBMITTER: Stoops EH 

PROVIDER: S-EPMC5123909 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature