Noninvasive detection of enzyme activity in tumor models of human ovarian cancer using catalyCEST MRI.

ABSTRACT:

Purpose

We proposed to detect the in vivo enzyme activity of γ-glutamyl transferase (GGT) within mouse models of human ovarian cancers using catalyCEST MRI with a diamagnetic CEST agent.

Methods

A CEST-FISP MRI protocol and a diamagnetic CEST agent were developed to detect GGT enzyme activity in biochemical solution. A quantitative Michaelis-Menten enzyme kinetics study was performed to confirm that catalyCEST MRI can measure enzyme activity. In vivo catalyCEST MRI studies generated pixel-wise activity maps of GGT activities. Ex vivo fluorescence imaging was performed for validation.

Results

CatalyCEST MRI selectively detected two CEST signals from a single CEST agent, whereby one CEST signal was responsive to GGT enzyme activity and the other CEST signal was an unres

SUBMITTER: Sinharay S 

PROVIDER: S-EPMC5123981 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature