Fluorescence chromosome banding and FISH mapping in perennial ryegrass, Lolium perenne L.

ABSTRACT:

Background

The unambiguous identification of individual chromosomes is a key part of the genomic characterization of any species. In this respect, the development and application of chromosome banding techniques has revolutionised mammalian and especially, human genomics. However, partly because of the traditional use of chromosome squash preparations, consistent fluorescence banding has rarely been achieved in plants. Here, successful fluorescence chromosome banding has been achieved for the first time in perennial ryegrass (Lolium perenne), a forage and turf grass with a large genome and a symmetrical karyotype with chromosomes that are difficult to distinguish.

Results

Based on flame-dried chromosome preparations instead of squashes, a simple fluorescence Q-banding techni

SUBMITTER: Ansari HA 

PROVIDER: S-EPMC5124321 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature