N-acetylcysteine Protects Mice from High Fat Diet-induced Metabolic Disorders.

ABSTRACT:

Purpose

To study the effects of N-acetylcysteine (NAC, C5H9NO3S) on diet-induced obesity and obesity-related metabolic disorders.

Methods

Six-week-old male C57BL/6 mice fed a chow or high-fat diet (HFD) were treated with NAC (2 g/L) in drinking water for 11 weeks. Its influences on body weight and food intake were manually measured, and influence on body composition were analyzed by magnetic residence imaging. Glucose meter and ELISA were used to determine serum glucose and insulin levels, as well as lipid content in the liver. The effects of NAC treatment on mRNA levels of genes involved in inflammation, thermogenesis, and lipid metabolism in various tissues were determined by real time PCR.

Results

NAC supplementation inhibited the increase of fat mass and the devel

SUBMITTER: Ma Y 

PROVIDER: S-EPMC5124332 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature