CagY Is an Immune-Sensitive Regulator of the Helicobacter pylori Type IV Secretion System.

ABSTRACT:

Background & aims

Peptic ulcer disease and gastric cancer are caused most often by Helicobacter pylori strains that harbor the cag pathogenicity island, which encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into host cells. cagY is an essential gene in the T4SS and has an unusual DNA repeat structure that predicts in-frame insertions and deletions. These cagY recombination events typically lead to a reduction in T4SS function in mouse and primate models. We examined the role of the immune response in cagY-dependent modulation of T4SS function.

Methods

H pylori T4SS function was assessed by measuring CagA translocation and the capacity to induce interleukin (IL)8 in gastric epithelial cells. cagY recombination was determined by changes in polymeras

SUBMITTER: Barrozo RM 

PROVIDER: S-EPMC5124400 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature