Brain microbiota disruption within inflammatory demyelinating lesions in multiple sclerosis.

ABSTRACT: Microbial communities reside in healthy tissues but are often disrupted during disease. Bacterial genomes and proteins are detected in brains from humans, nonhuman primates, rodents and other species in the absence of neurological disease. We investigated the composition and abundance of microbiota in frozen and fixed autopsied brain samples from patients with multiple sclerosis (MS) and age- and sex-matched nonMS patients as controls, using neuropathological, molecular and bioinformatics tools. 16s rRNA sequencing revealed Proteobacteria to be the dominant phylum with restricted diversity in cerebral white matter (WM) from MS compared to nonMS patients. Both clinical groups displayed 1,200-1,400 bacterial genomes/cm³ and low bacterial rRNA:rDNA ratios in WM. RNAseq analyses sho

SUBMITTER: Branton WG 

PROVIDER: S-EPMC5125007 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature